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quickblocktm blocking buffer for immunostaining  (Beyotime)


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    Beyotime quickblocktm blocking buffer for immunostaining
    Quickblocktm Blocking Buffer For Immunostaining, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quickblocktm+blocking+buffer+for+immunostaining/quickblocktm+blocking+buffer+for+immunol+staining/pm40141200-222-27-43
    Average 90 stars, based on 1 article reviews
    quickblocktm blocking buffer for immunostaining - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Foxa1 mediates eccrine sweat gland development through transcriptional regulation of Na-K-ATPase expression
    Article Snippet: .. The samples were blocked with QuickBlockTM Blocking Buffer for Immunostaining (Beyotime) for 10 min, labelled with rabbit anti-Foxa1 antibody (Abcam, 1:400 dilution, UK), mouse monoclonal anti-NKA α2 (1:200 dilution, Santa Cruz, USA) for 8 h at 4°C, and then incubated with Alexa Fluor 546-labeled anti-rabbit IgG (H+L) secondary antibodies (1:500 dilution, Abcam) for 2 h in the dark. .. For counterstaining, the sections were incubated with 4′,6-diamidino-2-phenylindole (DAPI, Beyotime) in the dark for 1 min at room temperature.

    Article Title: Direct reprogramming of human fibroblasts into hair-inducing dermal papilla cell-like cells by a single small molecule.
    Article Snippet: Dermal papilla cells (DPCs) are a crucial subset of mesenchymal cells in the skin responsible for regulating hair follicle development and growth, making them invaluable for cell-based therapies targeting hair loss.. However, obtaining sufficient DPCs with potent hair-inducing abilities remains a persistent challenge.. In this study, the Food and Drug Administration (FDA)-approved drug library was utilized to screen small molecules capable of reprogramming readily accessible human skin fibroblasts into functional DPCs.

    Article Title: EGCG drives gut microbial remodeling-induced epithelial GPR43 activation to lessen Th1 polarization in colitis.
    Article Snippet: .. After washing twice with PBS, cells were fixed, permeabilized, and blocked using quickblockTM blocking buffer for immunostaining (Beyotime, China). ..

    Article Title: A Single-Cell Landscape of Spermioteleosis in Mice and Pigs
    Article Snippet: .. The sections were finally incubated in citrate antigen retrieval solution (pH 6.0) at 96 °C for 10 min. As the sections gradually cooled, they were blocked in QuickBlockTM Blocking Buffer for Immunostaining (Beyotime, Nantong, China) for 10 min and incubated with primary antibodies at 4 °C overnight. .. Then, the sections were incubated with secondary antibodies for 30 min at 37 °C, washed with PBST after incubation with antibodies, and incubated with Antifade Mounting Medium containing DAPI (Beyotime, Nantong, China).

    Article Title: Baicalin prevents fibrosis of human trabecular meshwork cells via inhibiting the MyD88/NF-κB pathway.
    Article Snippet: Trabecular meshwork fibrosis contributes to increased aqueous humor outflow resistance, leading to elevated intraocular pressure in primary open-angle glaucoma.. Baicalin, an extract from Scutellaria baicalensis Georgi, has shown anti-fibrotic effects in liver, lung, and kidney diseases.. However, its anti-fibrotic effect on human trabecular meshwork (HTM) cells has not yet been clarified.

    Article Title: Fibroblast growth factor 21 alleviates unilateral ureteral obstruction-induced renal fibrosis by inhibiting Wnt/β-catenin signaling pathway.
    Article Snippet: Fibroblast growth factor 21 (FGF21) is a key regulator of energy metabolism.. Recent studies suggested that serum FGF21 levels increase with declining renal function.. However, the link between FGF21 and kidney diseases and the direct effect of FGF21 in renal fibrosis remains unclear.

    Article Title: Lanatoside C Inhibits Proliferation and Induces Apoptosis in Human Prostate Cancer Cells Through the TNF/IL-17 Signaling Pathway.
    Article Snippet: .. The Cell Counting Kit-8 (CCK-8), cell cycle detection kit, DAB horseradish peroxidase color development kit, enhanced immunostaining permeabilization buffer, 4% paraformaldehyde fix solution, QuickBlockTM blocking buffer for immunostaining staining, and QuickBlockTM primary and secondary antibody dilution buffer for immunohistochemistry were all purchased from Beyotime Biotechnology (Shanghai, China). .. AnnexinV-FITC/PI kits were obtained from Yeasen (Shanghai, China).

    Article Title: TET2 regulates osteoclastogenesis by modulating autophagy in OVX-induced bone loss.
    Article Snippet: .. Then, 4% paraformaldehyde (New Cell & Molecular Biotechnology, N1012), 0.2% Triton X-100 (Beyotime, P0096) and QuickBlockTM blocking buffer for immunostaining (Beyotime, P0102) were used to fix, permeabilize and seal the cells, respectively, in sequence. .. Then, the cells were incubated with anti-LC3B (Abcam, ab192890; 1 μg/ ml) antibodies, goat anti-rabbit IgG H&L (Abcam, Alexa Fluor 647; 1:500), and DAPI (Beyotime, C1006) successively.

    Immunostaining:

    Article Title: Foxa1 mediates eccrine sweat gland development through transcriptional regulation of Na-K-ATPase expression
    Article Snippet: .. The samples were blocked with QuickBlockTM Blocking Buffer for Immunostaining (Beyotime) for 10 min, labelled with rabbit anti-Foxa1 antibody (Abcam, 1:400 dilution, UK), mouse monoclonal anti-NKA α2 (1:200 dilution, Santa Cruz, USA) for 8 h at 4°C, and then incubated with Alexa Fluor 546-labeled anti-rabbit IgG (H+L) secondary antibodies (1:500 dilution, Abcam) for 2 h in the dark. .. For counterstaining, the sections were incubated with 4′,6-diamidino-2-phenylindole (DAPI, Beyotime) in the dark for 1 min at room temperature.

    Article Title: Direct reprogramming of human fibroblasts into hair-inducing dermal papilla cell-like cells by a single small molecule.
    Article Snippet: Dermal papilla cells (DPCs) are a crucial subset of mesenchymal cells in the skin responsible for regulating hair follicle development and growth, making them invaluable for cell-based therapies targeting hair loss.. However, obtaining sufficient DPCs with potent hair-inducing abilities remains a persistent challenge.. In this study, the Food and Drug Administration (FDA)-approved drug library was utilized to screen small molecules capable of reprogramming readily accessible human skin fibroblasts into functional DPCs.

    Article Title: EGCG drives gut microbial remodeling-induced epithelial GPR43 activation to lessen Th1 polarization in colitis.
    Article Snippet: .. After washing twice with PBS, cells were fixed, permeabilized, and blocked using quickblockTM blocking buffer for immunostaining (Beyotime, China). ..

    Article Title: A Single-Cell Landscape of Spermioteleosis in Mice and Pigs
    Article Snippet: .. The sections were finally incubated in citrate antigen retrieval solution (pH 6.0) at 96 °C for 10 min. As the sections gradually cooled, they were blocked in QuickBlockTM Blocking Buffer for Immunostaining (Beyotime, Nantong, China) for 10 min and incubated with primary antibodies at 4 °C overnight. .. Then, the sections were incubated with secondary antibodies for 30 min at 37 °C, washed with PBST after incubation with antibodies, and incubated with Antifade Mounting Medium containing DAPI (Beyotime, Nantong, China).

    Article Title: Baicalin prevents fibrosis of human trabecular meshwork cells via inhibiting the MyD88/NF-κB pathway.
    Article Snippet: Trabecular meshwork fibrosis contributes to increased aqueous humor outflow resistance, leading to elevated intraocular pressure in primary open-angle glaucoma.. Baicalin, an extract from Scutellaria baicalensis Georgi, has shown anti-fibrotic effects in liver, lung, and kidney diseases.. However, its anti-fibrotic effect on human trabecular meshwork (HTM) cells has not yet been clarified.

    Article Title: Fibroblast growth factor 21 alleviates unilateral ureteral obstruction-induced renal fibrosis by inhibiting Wnt/β-catenin signaling pathway.
    Article Snippet: Fibroblast growth factor 21 (FGF21) is a key regulator of energy metabolism.. Recent studies suggested that serum FGF21 levels increase with declining renal function.. However, the link between FGF21 and kidney diseases and the direct effect of FGF21 in renal fibrosis remains unclear.

    Article Title: Lanatoside C Inhibits Proliferation and Induces Apoptosis in Human Prostate Cancer Cells Through the TNF/IL-17 Signaling Pathway.
    Article Snippet: .. The Cell Counting Kit-8 (CCK-8), cell cycle detection kit, DAB horseradish peroxidase color development kit, enhanced immunostaining permeabilization buffer, 4% paraformaldehyde fix solution, QuickBlockTM blocking buffer for immunostaining staining, and QuickBlockTM primary and secondary antibody dilution buffer for immunohistochemistry were all purchased from Beyotime Biotechnology (Shanghai, China). .. AnnexinV-FITC/PI kits were obtained from Yeasen (Shanghai, China).

    Article Title: TET2 regulates osteoclastogenesis by modulating autophagy in OVX-induced bone loss.
    Article Snippet: .. Then, 4% paraformaldehyde (New Cell & Molecular Biotechnology, N1012), 0.2% Triton X-100 (Beyotime, P0096) and QuickBlockTM blocking buffer for immunostaining (Beyotime, P0102) were used to fix, permeabilize and seal the cells, respectively, in sequence. .. Then, the cells were incubated with anti-LC3B (Abcam, ab192890; 1 μg/ ml) antibodies, goat anti-rabbit IgG H&L (Abcam, Alexa Fluor 647; 1:500), and DAPI (Beyotime, C1006) successively.

    Incubation:

    Article Title: Foxa1 mediates eccrine sweat gland development through transcriptional regulation of Na-K-ATPase expression
    Article Snippet: .. The samples were blocked with QuickBlockTM Blocking Buffer for Immunostaining (Beyotime) for 10 min, labelled with rabbit anti-Foxa1 antibody (Abcam, 1:400 dilution, UK), mouse monoclonal anti-NKA α2 (1:200 dilution, Santa Cruz, USA) for 8 h at 4°C, and then incubated with Alexa Fluor 546-labeled anti-rabbit IgG (H+L) secondary antibodies (1:500 dilution, Abcam) for 2 h in the dark. .. For counterstaining, the sections were incubated with 4′,6-diamidino-2-phenylindole (DAPI, Beyotime) in the dark for 1 min at room temperature.

    Article Title: A Single-Cell Landscape of Spermioteleosis in Mice and Pigs
    Article Snippet: .. The sections were finally incubated in citrate antigen retrieval solution (pH 6.0) at 96 °C for 10 min. As the sections gradually cooled, they were blocked in QuickBlockTM Blocking Buffer for Immunostaining (Beyotime, Nantong, China) for 10 min and incubated with primary antibodies at 4 °C overnight. .. Then, the sections were incubated with secondary antibodies for 30 min at 37 °C, washed with PBST after incubation with antibodies, and incubated with Antifade Mounting Medium containing DAPI (Beyotime, Nantong, China).

    Article Title: Fibroblast growth factor 21 alleviates unilateral ureteral obstruction-induced renal fibrosis by inhibiting Wnt/β-catenin signaling pathway.
    Article Snippet: Fibroblast growth factor 21 (FGF21) is a key regulator of energy metabolism.. Recent studies suggested that serum FGF21 levels increase with declining renal function.. However, the link between FGF21 and kidney diseases and the direct effect of FGF21 in renal fibrosis remains unclear.

    Saline:

    Article Title: Baicalin prevents fibrosis of human trabecular meshwork cells via inhibiting the MyD88/NF-κB pathway.
    Article Snippet: Trabecular meshwork fibrosis contributes to increased aqueous humor outflow resistance, leading to elevated intraocular pressure in primary open-angle glaucoma.. Baicalin, an extract from Scutellaria baicalensis Georgi, has shown anti-fibrotic effects in liver, lung, and kidney diseases.. However, its anti-fibrotic effect on human trabecular meshwork (HTM) cells has not yet been clarified.

    CCK-8 Assay:

    Article Title: Lanatoside C Inhibits Proliferation and Induces Apoptosis in Human Prostate Cancer Cells Through the TNF/IL-17 Signaling Pathway.
    Article Snippet: .. The Cell Counting Kit-8 (CCK-8), cell cycle detection kit, DAB horseradish peroxidase color development kit, enhanced immunostaining permeabilization buffer, 4% paraformaldehyde fix solution, QuickBlockTM blocking buffer for immunostaining staining, and QuickBlockTM primary and secondary antibody dilution buffer for immunohistochemistry were all purchased from Beyotime Biotechnology (Shanghai, China). .. AnnexinV-FITC/PI kits were obtained from Yeasen (Shanghai, China).

    Staining:

    Article Title: Lanatoside C Inhibits Proliferation and Induces Apoptosis in Human Prostate Cancer Cells Through the TNF/IL-17 Signaling Pathway.
    Article Snippet: .. The Cell Counting Kit-8 (CCK-8), cell cycle detection kit, DAB horseradish peroxidase color development kit, enhanced immunostaining permeabilization buffer, 4% paraformaldehyde fix solution, QuickBlockTM blocking buffer for immunostaining staining, and QuickBlockTM primary and secondary antibody dilution buffer for immunohistochemistry were all purchased from Beyotime Biotechnology (Shanghai, China). .. AnnexinV-FITC/PI kits were obtained from Yeasen (Shanghai, China).

    Immunohistochemistry:

    Article Title: Lanatoside C Inhibits Proliferation and Induces Apoptosis in Human Prostate Cancer Cells Through the TNF/IL-17 Signaling Pathway.
    Article Snippet: .. The Cell Counting Kit-8 (CCK-8), cell cycle detection kit, DAB horseradish peroxidase color development kit, enhanced immunostaining permeabilization buffer, 4% paraformaldehyde fix solution, QuickBlockTM blocking buffer for immunostaining staining, and QuickBlockTM primary and secondary antibody dilution buffer for immunohistochemistry were all purchased from Beyotime Biotechnology (Shanghai, China). .. AnnexinV-FITC/PI kits were obtained from Yeasen (Shanghai, China).

    Sequencing:

    Article Title: TET2 regulates osteoclastogenesis by modulating autophagy in OVX-induced bone loss.
    Article Snippet: .. Then, 4% paraformaldehyde (New Cell & Molecular Biotechnology, N1012), 0.2% Triton X-100 (Beyotime, P0096) and QuickBlockTM blocking buffer for immunostaining (Beyotime, P0102) were used to fix, permeabilize and seal the cells, respectively, in sequence. .. Then, the cells were incubated with anti-LC3B (Abcam, ab192890; 1 μg/ ml) antibodies, goat anti-rabbit IgG H&L (Abcam, Alexa Fluor 647; 1:500), and DAPI (Beyotime, C1006) successively.



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